human tongue squamous carcinoma cell lines scc25  (ATCC)

Journal: International journal of molecular sciences

Article Title: Nuciferine Inhibits Oral Squamous Cell Carcinoma Partially through Suppressing the STAT3 Signaling Pathway.

doi: 10.3390/ijms241914532

Figure Lengend Snippet: Figure 1. Nuciferine (NF) inhibited the proliferation and promoted the apoptosis of OSCC cells. (A) The growth-inhibiting effects of NF (100 µM, 24 h) and the cytotoxicity test of NF (0~100 µM, 24 h) on OSCC cells were detected by CCK–8. (B) The viability of SCC25 and CAL27 cells was inhibited by NF (0~120 µM, 0~96 h) in a dose-dependent manner, as verified by CCK–8. (C–F) The proliferation of OSCC cells was suppressed by NF, as demonstrated by the colony formation assay (80 µM, 7 d) and EdU assay (80 µM, 24 h). (G) Increased expression of pro-apoptosis markers Bax and Caspase 3, and decreased expression of anti-apoptosis marker Bcl-2 in OSCC cells treated with NF (80 µM, 48 h) were detected by western blot. (H,I) The apoptosis-promoting effects of NF (80 µM, 48 h) on OSCC cells were verified by flow cytometry. ns, not significant. * p < 0.05, ** p < 0.01, *** p < 0.001 and *** p < 0.0001.

Article Snippet: For this research, the human tongue squamous cell carcinoma cell lines SCC25 and CAL27 were initially purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: CCK-8 Assay, Colony Assay, EdU Assay, Expressing, Marker, Western Blot, Cytometry

Journal: Bioscience Reports

Article Title: Identification of CCT3 as a prognostic factor and correlates with cell survival and invasion of head and neck squamous cell carcinoma

doi: 10.1042/BSR20211137

Figure Lengend Snippet: ( A ) CERES score was obtained from Depmap datasource and used to access the significance of CCT3 for cell survival of HNSCC. CERES score approach to -1 means the gene is important for cell survival, while score approach to 0 means the gene is not an essential gene. ( B ) SCC25 and CAL27 cells were transfected with control siRNA or siRNA-CCT3 for 48 h. Cells were harvested and subjected to Western blot assay to detect the expression of CCT3. A CCK-8 assay was performed to detect cell viability of SCC25 ( C ) and CAL27 ( D ) cells after being transfected with siRNA. ( E ) A wound healing assay was performed to determine the cell invasion of SCC25 and CAL27 cells after being transfected with siRNA. The cells were photographed at 0 or 48 h; *, P < 0.05; **, P < 0.01; ***, P < 0.001; CCT3, Chaperonin-containing TCP-1 3.

Article Snippet: The human tongue squamous cell carcinoma cell lines SCC25 and CAL27 were kindly provided by Prof. Juan Lv (Taihe Hospital, Shiyan) [ ].

Techniques: Transfection, Control, Western Blot, Expressing, CCK-8 Assay, Wound Healing Assay

Journal: Nanomedicine : nanotechnology, biology, and medicine

Article Title: Coassembled nanostructured bioscaffold reduces the expression of proinflammatory cytokines to induce apoptosis in epithelial cancer cells.

doi: 10.1016/j.nano.2016.01.009

Figure Lengend Snippet: Figure 2. Biocompatability and the effect of the material on Cancer (SCC25) and healthy (hMFC) cell fate. A) the shows the relative viabilities of the cells on each scaffold over three days. SCC25 cells were seeded on the Fmoc-FRGDF hydrogel (0RGD) and 2 mg/mL fucoidan (2RGD) and incubated for 3 days. Calcein AM staining was performed to identify live cells (green) and propidium iodide was used to identify dead cells (red). Scale bars 125μm. A minimum of 5 fields were captured for each treatment and number of live cells and dead cells were counted individually and percentage was calculated * p = 0.05, ** p = 0.01, *** p = 0.001 **** p= 0.0001. (n=3) B). shows the distribution of SCC25 cells on day 3 on each hydrogel C and D) Apoptosis at 24 and 48 hours. In order to determine the mode of cell death in the SCC25 cells, we stained the cells using Annexin V (green), Propidium iodide (red) and Hoechst dye (blue). Live cells are identified by a nucleus stained only with hoechst dye and appear only blue Early apoptotic cells were stained green with nucleus blue and the late apoptotic cells stained in green with a red nucleus. Scale bars 50μm.

Article Snippet: AC C EP TE D M AN U SC R IP T Cell lines and culture conditions: The human tongue squamous cell carcinoma cell line (SCC25) cultures were obtained verified from ATCC and were maintained in DMEM-F12 complete medium containing 10% fetal bovine serum, 400 ng/mL hydrocortisone and pencillin/streptomycin.

Techniques: Incubation, Staining

Journal: Nanomedicine : nanotechnology, biology, and medicine

Article Title: Coassembled nanostructured bioscaffold reduces the expression of proinflammatory cytokines to induce apoptosis in epithelial cancer cells.

doi: 10.1016/j.nano.2016.01.009

Figure Lengend Snippet: Figure 5. Co-assembled hydrogels inhibits the expression of proinflammatory cytokines and disrupts cell division on a gene and protein level. A) Visualisation of protein expresion of fluorescent NFκβ p65 and CEP55 immunofluorescence analysis (green) in SCC25 cells localised with a nuclear counterstain of Hoechst dye (blue) cultured on the hydrogels for 48 h B) shows NFκβ p65 in the nucleus increasing with 10 g/mL of LPS stimulation in 0RGD cultures, whereas CEP55 expression was high in SCC25 cells irrespective of stimulation with LPS. Both signals were significantly reduced in cells cultured on the 2RGD hydrogels. Scale bars 25μm. C) Gene expression profiles of the pro-inflammatory cytokine response elements in LPS stimulated and non-stimulated SCC25 cells as determined by qPCR. Interestingly, in each case, gene expression was reduced on the 2RGD hydrogels compared with 0RGD. When LPS was used to stimulate the pro-inflammatory pathway, the expression increased significantly on 0RGD in each case, but remained comparable to the unchallenged cells on 2RGD. * p = 0.05, ** p = 0.01, *** p = 0.001 **** p= 0.0001

Article Snippet: AC C EP TE D M AN U SC R IP T Cell lines and culture conditions: The human tongue squamous cell carcinoma cell line (SCC25) cultures were obtained verified from ATCC and were maintained in DMEM-F12 complete medium containing 10% fetal bovine serum, 400 ng/mL hydrocortisone and pencillin/streptomycin.

Techniques: Expressing, Immunofluorescence, Cell Culture, Gene Expression